ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 35, Number 4
JKCSEZ 35(4)
August 20, 1991 

The electrochemical reduction behavior of Bilirubin (BR) in phosphate buffer (pH 7.8) solution was studied by DC polarography, differential pulse polarography, cyclic voltammetry and controlled potential coulometry. In DC polarogram, two reduction waves of BR were found. The half wave potentials of two reduction waves were -1.32 and -1.51 volts vs. Ag/AaCl respectively. The current type of 1st reduction wave was diffusion-controlled and the 2nd reduction wave was diffusion current containing a little kinetic current. The electrochemical reduction process of BR at each reduction step was all irreversible. The prewave appeared at lower concentration than 3.4 × 10-4M, this prewave was identified as adsorption prewave. And the number of electron transfered in reduction steps, napp was two for the 1st reduction step and one for the 2nd reduction step.