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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 35, Number 10
BKCSDE 35(10)
October 20, 2014 

Selective Extraction and Quantification of Glutathione using Maleimide-Presenting Gold Nanoparticles
Hongseok Oh, Jeongwook Lee, Woon-Seok Yeo*
Glutathione, Quantification, Gold Nanoparticles, MALDI-TOF MS, Internal standard
In this paper, we describe a new method for the selective extraction and quantification of glutathione (GSH) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and maleimide-presenting gold nanoparticles (Mal-AuNPs). Our strategy utilizes the Michael addition to selectively extract GSH, from chosen samples, onto the maleimide of Mal-AuNPs. After the extraction step, the GSH bound to the AuNPs was analyzed by MALDI-TOF MS in the presence of an internal standard which was prepared by reacting Mal-AuNPs with isotope-labeled GSH (GSH*). The GSH* has the same structure as GSH but a higher molecular weight, and therefore, enables absolute quantification of GSH by comparing the mass signal intensities of the GSH- and GSH*-conjugated alkanethiols. Our strategy was verified by analyzing GSHspiked fetal bovine serum and NIH 3T3 cells.
3047 - 3051
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