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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 35, Number 7
BKCSDE 35(7)
July 20, 2014 

An Image Cytometric MTT Assay as an Alternative Assessment Method of Nanoparticle Cytotoxicity
Song Hee Lee, Jonghoon Park, Dongwook Kwon, Tae Hyun Yoon*
Alternative assay method, Nanoparticles cytotoxicity, Image cytometry, MTT assay, TiO2 nanoparticles
Despite increasing importance of in vitro cell-based assays for the assessment of nanoparticles (NPs) cytotoxicity, their suitability for the assessment of NPs toxicity is still in doubt. Here, limitations of widely used cell viability assay protocol (i.e., MTT asssay) for the cytotoxicity assessment of P25 TiO2 NPs were carefully examined and an alternative toxicity assessment method to overcome these limitations was proposed, where the artifacts caused by extracellularly formed formazan and light scattered by agglomerated NPs were minimized by measuring only the intracellular formazan via image cytometric methods.
1933 - 1938
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