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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 31, Number 2
BKCSDE 31(2)
February 20, 2010

Title	An NMR Study on the Phase Change of Lipid Membranes by an Antimicrobial Peptide, Protegrin-1
Author	Chul Kim
Keywords	Antimicrobial peptide, Protegrin-1, Toroidal pore, ²H solid-state NMR, Phase change
Abstract	Membrane disruption by an antimicrobial peptide, protegrin-1 (PG-1), was investigated by measuring the ²H solid-state nuclear magnetic resonance (SSNMR) spectra of 1-palmitoyl-d₃₁-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC_ d31) in the mixture of PG-1 and POPC_d₃₁ lipids deposited on thin cover-glass plates. The experimental line shapes of anisotropic ²H SSNMR spectra measured at various peptide-to-lipid (P/L) ratios were simulated reasonably by assuming the mosaic spread of bilayers containing pore structures or the coexistence of the mosaic spread of bilayers and a fast-tumbling isotropic phase. Within a few days of incubation in the hydration chamber, the pores were formed by the peptide in the POPC_d₃₁ and POPC_d₃₁/cholesterol membranes. However, the formation of the pores was not clear in the POPC_d₃₁/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol (POPG) membrane. Over a hundred days after hydration, a rapidly rotating isotropic phase increased in the POPC_d₃₁ and the POPC_d₃₁/cholesterol membranes with the higher P/L ratios, but no isotropic phase appeared in the POPC_d₃₁/POPG membrane. Cholesterol added in the POPC bilayer acted as a stabilizer of the pore structure and suppressed the formation of a fast-tumbling isotropic phase.
Page	372 - 378
Full Text	PDF

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