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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 27, Number 11
BKCSDE 27(11)
November 20, 2006

Title	Purification and Characterization of [Ala²]-Neuromedin N from the Visceral Tissue of the African Lungfish, Protopterus dolloi
Author	Chan-Hee Kim, Hye-Jin Go, Eun Jung Kim, Jung-Kil Seo, Yong-Ki Hong, Hyung-Rak Kim, Joon-Ki Chung, Yojiro Muneoka, Nam Gyu Park*
Keywords	African lungfish, Purification, [Ala²]-Neuromedin N
Abstract	A new biologically active peptide with structural similarity to neuromedin N (NMN) has been isolated from extracts of visceral tissue of the African lungfish, Protopterus dolloi, using the rectum of the quail as the bioassay system. The primary structure of NMN-related peptide was established as Lys-Ala-Pro-Tyr-Ile-Leu-OH ([Ala²]-NMN) and contained one substitution (Ala² → Ile) compared with the porcine NMN. [Ala²]-NMN was found to have an excitatory effect on rectal muscle tissues of quail (Coturnix japonica), newt (Cynops pyrrhogaster) and black bass (Micropterus sulmoides). The threshold concentration of [Ala²]-NMN for contraction of C. japonica muscle was found to be approximately 10^-11 M. [Ala²]-NMN showed contractile activities in the following order: C. japonica > C. pyrrhogaster > M. sulmoides. The identification of [Ala²]-NMN provides evidence that NMN family, hitherto confined to mammals, has a widespread occurrence in lungfish.
Page	1733 - 1736
Full Text	PDF

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