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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 40, Number 3
JKCSEZ 40(3)
June 20, 1996 

Observation of Effects of Phosphatidylcholine and Al Salts Concentration on the Formation of Vesicles by TEM

베시클 생성에 미치는 Phosphatidylcholine과 알루미늄 염 농도의 영향에 관한 TEM 관찰
Jong-Jae Chung, Chang-Hyun Kim, Byung-Kyo Lee, Hae-Wook Lee

투과전자현미경(TEM)을 사용하여 실험 변수에 따른 베시클의 크기와 베시클간의 응집을 관찰하였다. 조절된 변수로는 (a) 반응온도의 변화, (b) phosphatidylcholine 농도의 변화, (c) phosphatidylcholine의 농도(0.39mM)를 일정하게 유지했을 대 알루미늄 이온의 농도 변화: 0.01, 0.05, 0.1, 0.2 M, (d)Al(NO3)3ㆍ9H2O에 phosphatidylcholine을 1wt%로 고정하여 이들의 농도 증가에 따른 전체농도의 변화 등을 실험한 결과 베시클 크기와 베시클간의 응집이 관찰되었다. 반응온도가 증가할수록, phosphatidylcholine 농도가 증가할수록 베시클의 크기가 감소되었다. 알루미늄 이온농도가 0.2M일 때 입자간의 응집과 합체로 비정상 베시클 성장이 관찰되었다. Al(NO3)3ㆍ9H2O에 대한 phosphatidylcholine비가 1 wt%일 때 최적의 베시클 크기와 균일한 분포를 얻을 수 있다.

Transmission Electron Microscopy (TEM) was used to monitor the response of vesicle size and the intervesicle agglomeration with a variety of experimental parameters. Considered parameters are: (a) reaction temperture, (b) concentration of phosphatidylcholine, (c) concentration of aluminum ion with fixed concentration of phosphatidylcholine at 0.39 mM, and (d) mixed concentration of aluminum and phosphatidylcholine when fixing the weight ratio of phosphatidylcholine to aluminum at 0.01. Controlling these parameters, vesicle size changed and intervesicle agglomeration observed. As reaction temperature and the concentration of phosphatidylcholine increase, vesicle size decreases. With 0.2 M of aluminum ion, abnormal vesicle growth led by intervesicle agglomeration and coalescence was observed. When weight ratio of phosphatidylcholine to aluminum is 0.01, optimal vesicle size and size distribution can be obtained.

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