ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 37, Number 6
JKCSEZ 37(6)
December 20, 1993 

Supercritical Fluid Chromatography(SFC) has become a technique for solving problems that are difficult by other chromatographic methods. However, the most widely used fluid, is no more polar than hexane. Polar samples which are difficult to be analyzed with pure supercritical CO2 because of their high polarity can be separated by adding polar modifiers to supercritical CO2. In this paper, a new method for monitering the mobile phase composition in modified supercritical fluid chromatography was developed. The amount of water dissolved in supercritical CO2 was measured by amperometric microsensor which is made of thin film of perfluorosulfonate ionomer (PFSI). The amount of water dissolved in supercritical CO2 stayed constant for a much longer time than with a saturator column. With this new mixing device, we could do good separations for insecticides and fungicides which are difficult to separate with pure CO2.