ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 35, Number 6
JKCSEZ 35(6)
December 20, 1991 

Metachromatic behavior of methylene blue (MB) in solutions of aqueous sodium dodecyl sulfate (SDS), chondroitin sulfate and L-α-lecithin vesicle at 18~52℃ has been studied by absorption spectroscopy. MB was clustered in the matrix of the vesicle with high concentration of L-α-lecithin. The metachromasy of MB was found to be independent of phase transition temperature of vesicles. These results suggest that the dyes were aggregated on the hydrophilic surfaces of vesicle. In the vesicular system, the metachromatic effect of MB was dramatically decreased in the presence of hexadecyltrimethylammonium bromide (CTAB) than SDS. It is estimated that the intercalation site of these surfactants on vesicle surfaces was different, that is, the intercalation of CTAB was more effective than that of SDS.