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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 57, Number 3
JKCSEZ 57(3)
June 20, 2013 

Application of Pine Peroxidase to the Amperometric Determination of Hydrogen Peroxidase

과산화수소의 전류법적 정량을 위한 소나무 과산화효소의 활용
Kil Joong Yoon*

Enzyme electrode, Pine peroxidase, Hydrogen peroxide, Mediator
A pine needle-embedded graphite enzyme electrode, of which bonding agent is CSM rubber, was newly designed and its electrochemistry was studied based on the amperometry. It involved a ground green leaves of pine tree as a zymogen together with electrochemical mediator, ferrocene within the paste. The plots of ln(i(1-enfη)) vs. η and Lineweaver- Burk at the low potential (-100 to -500 mV) showed good linearities indicating that the amperometric response is by the catalytic power of pine peroxidase. Electrochemical parameters obtained, symmetry factor (α, 0.17), limiting current (il, 1.99 A/ cm2), exchange current density (i0, 5.86×10-5 A/cm2), Michaelis constant (KM, 1.68×10-3 M) and many others showed that pine peroxidase discharges the role of catalyst quantitatively on the electrode surface. Those proved that the practical use of pine peroxidase is promising in place of the marketed.
329 - 334
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