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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 56, Number 5
JKCSEZ 56(5)
October 20, 2012 

Development of HRP-modified Carbon Composite Biosensor and Electrochemical Analysis of H2O2

Horseradish peroxidase가 변성된 탄소복합 바이오센서 개발 및 전기화학적 H2O2분석
Deog Su Park*

Sol-gel, Carbon composite, Horseradish peroxidase, Biosensor, Hydrogen peroxide
A sol-gel derived carbon composite electrodes (CCEs) were fabricated by mixing horseradish peroxidase (HRP), sol of tetraethoxysilane (TESO), and graphite powder. The HRP solution was added to the sol solution of TEOS, and then graphite powder was added to this mixture. The resulting carbon ceramic network effectively encapsulated HRP and shows a catalytic reduction starting at -0.2 V for H2O2. The optimum conditions for H2O2 determination have been characterized with respect to the enzyme loading ratio and pH. The linear range and detection limit of H2O2 detection were from 0.2 mM to 2.2 mM and 0.035 mM, respectively. The common electroactive interferences such as ascorbic acid, acetaminophene, and uric acid were not affected upon the response to H2O2 at the HRP biosensor due to low detection potential.
571 - 576
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