ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 54, Number 6
JKCSEZ 54(6)
December 20, 2010 

Yeast is immobilized upon 100 μm CPG(controlled pore glass bead) to separate Sb³⁺ and Sb⁵⁺. Continuous hydride generation is performed after the bio-column. The optimum conditions are 0.8 M nitric acid as an eluent with the flow rate of 1.0 mL min^-1 and the optimum conditions for the generation of hydride are 2 M HCl, 3% (w/v) NaBH₄ with the flow rate of 0.83 mL min^-1, Ar carrier gas flow rate of 50 mL min^-1. Two species are separated at 112 and 354 seconds each. The sensitivity is enhanced by 10 times for 200 μL of sample and the detection limits are 3.0 ppb and 7.0 ppb for Sb³⁺ and Sb⁵⁺, respectively. When compared with the standard samples, this method showed accurate results.