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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 54, Number 6
JKCSEZ 54(6)
December 20, 2010 

Separation and Sensitive Determination of Sb Species using Yeast Bonded Bio-column with Continuous Hydride Generation

이이스트 고정 bio칼럼을 이용한 Sb의 화학종분리 및 연속적 수소화물발생법에 의한 감도개선
Jeong Ok Lee, Hyoshik Kwon, Yong Nam Pak*

이정옥, 권효식, 박용남*
안티몬 화학종, 이이스트 고정 bio컬럼, HPLC-수소화물 발생법 , Bio-column, Speciation of Sb, Yeast covalent bonded column, HPLC-Hydride generation

Yeast is immobilized upon 100 μm CPG(controlled pore glass bead) to separate Sb3+ and Sb5+. Continuous hydride generation is performed after the bio-column. The optimum conditions are 0.8 M nitric acid as an eluent with the flow rate of 1.0 mL min-1 and the optimum conditions for the generation of hydride are 2 M HCl, 3% (w/v) NaBH4 with the flow rate of 0.83 mL min-1, Ar carrier gas flow rate of 50 mL min-1. Two species are separated at 112 and 354 seconds each. The sensitivity is enhanced by 10 times for 200 μL of sample and the detection limits are 3.0 ppb and 7.0 ppb for Sb3+ and Sb5+, respectively. When compared with the standard samples, this method showed accurate results.

696 - 700
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