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ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 54, Number 5
JKCSEZ 54(5)
October 20, 2010 |
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Title |
Study of Interaction of Native DNA with Iron(III)-(2,4-Dihydroxysalophen)chloride
천연 DNA와 2,4-디히드록시살로펜-염화철(III)과 의 상호작용 연구
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Author |
Mohammad Reza Azani*, Azin Hassanpour, Abdol Khalegh Bordbar
Mohammad Reza Azani*, Azin Hassanpour, Abdol Khalegh Bordbar |
Keywords |
DNA, 쉬프염기, 상호작용, 형광, 자외선-가시광선 분광법
, DNA, Schiff base, Interaction, Fluorescence, UV-vis spectroscopy
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Abstract |
In this study, iron(III)-2,4-dihydroxysalophen chloride (Fe(2,4-DHSalophen)Cl), has been synthesized by combination of 2,4-dihydroxysalophen (2,4-DHSalophen) with FeCl2 in a solvent system. This complex combination was characterized using UV-vis and IR spectroscopies. Subsequently, the interaction between native calf thymus deoxyribonucleic acid (ct-DNA) and Fe(2,4-DHSalophen)Cl, was investigated in 10 mM Tris/HCl buffer solution, pH 7.2, using UV-visible absorption and fluorescence spectroscopies, thermal denaturation technique and viscosity measurements. From spectrophotometric titration experiments, the binding constant of Fe(2,4-DHSalophen)Cl with ct-DNA was found to be (1.6 ± 0.2) × 103 M-1. The fluorescence study represents the quenching effect of Fe(2,4-DHSalophen)Cl on bound ethidium bromide to DNA. The quenching process obeys linear Stern- Volmer equation in extended range of Fe(2,4-DHSalophen)Cl concentration. Thermal denaturation experiments represent the increasing melting temperature of DNA (about 4.3 oC) due to binding of Fe(2,4-DHSalophen)Cl. These results are consistent with a binding mode dominated by interactions with the groove of ct-DNA.
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Page |
573 - 578 |
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