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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 53, Number 3
JKCSEZ 53(3)
June 20, 2009 

 
Title
Preparation of Affinity Column Based on Zr4+ Ion for Phosphoproteins Isolation

Preparation of Affinity Column Based on Zr4+ Ion for Phosphoproteins Isolation
Author
Seon Mi Lee, In Ae Bae, Jung Hyen Park, Tae Dong Kim, Seong Ho Choi*

Seon Mi Lee, In Ae Bae, Jung Hyen Park, Tae Dong Kim, Seong Ho Choi*
Keywords
Zr4+ affinity column; poly(styrene-co-glycidyl methacrylate); polymer microsphere; phosphonated casein; resolution time , Zr4+ affinity column; poly(styrene-co-glycidyl methacrylate); polymer microsphere; phosphonated casein; resol
Abstract
This paper has described about preparation of Zr4+ affinity column based on the poly(styrene-co- glycidyl methacrylate) prepared by emulsion polymerization of styrene and glycidyl methacrylate in order to isolate phosphopeptide. The Zr4+ ions were introduced after the phophonation of an epoxy group on polymeric microspheres. The successful preparation of Zr4+-immobilized polymeric microsphere stationary phase was confirmed through Fourier transform infrared spectra, optical microscopy, scanning electron microscopy, X-ray photoelectron spectra and inductively coupled plasma-atomic emission spectrometer. The separation efficiency for Zr4+ affinity column prepared by slurry packing was tested to phosphonated casein and dephosphonated casein. The resolution time (min) of the phosphonated casein was higher than that of dephosphated casein for Zr4+ affinity polymeric microsphere by liquid chromatography. This Zr4+ affinity column can be used for isolation of phosphonated casein from casein using liquid chromatography.

This paper has described about preparation of Zr4+ affinity column based on the poly(styrene-co- glycidyl methacrylate) prepared by emulsion polymerization of styrene and glycidyl methacrylate in order to isolate phosphopeptide. The Zr4+ ions were introduced after the phophonation of an epoxy group on polymeric microspheres. The successful preparation of Zr4+-immobilized polymeric microsphere stationary phase was confirmed through Fourier transform infrared spectra, optical microscopy, scanning electron microscopy, X-ray photoelectron spectra and inductively coupled plasma-atomic emission spectrometer. The separation efficiency for Zr4+ affinity column prepared by slurry packing was tested to phosphonated casein and dephosphonated casein. The resolution time (min) of the phosphonated casein was higher than that of dephosphated casein for Zr4+ affinity polymeric microsphere by liquid chromatography. This Zr4+ affinity column can be used for isolation of phosphonated casein from casein using liquid chromatography.

Page
279 - 285
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