ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 49, Number 4
JKCSEZ 49(4)
August 20, 2005 

[³²P] γ-ATP (adenosine 5'-[γ-³²P]triphosphate) and [³²P] α-dCTP (deocycytidine 5'-[α-³²P]triphosphate) are representatives of P-32 labeled compounds and used as phosphorylating agents or labeled substrates for several RNA and DNA polymerase reactions. These are usually produced by enzymatic processing. Our studies have been focused mainly on preparing these compounds by chemical processing for mass production. O-8-(5-chloroquinolyl)-S-phenyl phosphorothiate, a coupling agent, is proper for the systhesis of [³²P] γ-ATP and [³²P] α-dCTP. Coupling agent has been prepared in a yield of 42%. [³²P] γ-ATP and [³²P] α-dCTP were prepared in radiochemical yields of 95% and 40%; respectively.