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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 49, Number 3
JKCSEZ 49(3)
June 20, 2005 
 
Title
 High Performance Liquid Chromatographic Determination of Homocysteine and Cystathionine in Biological Samples by Derivatization with 6-Aminoquinolyl-N-Hydroxylsuccinimidyl Carbamate (AQC)

High Performance Liquid Chromatographic Determination of Homocysteine and Cystathionine in Biological Samples by Derivatization with 6-Aminoquinolyl-N-Hydroxylsuccinimidyl Carbamate (AQC)
Author
 Seong S. Seo*

Seong S. Seo*
Keywords
 Homocysteine, Cystathionine, HPLC , Homocysteine, Cystathionine, HPLC
Abstract
 AQC를 사용하여 일차 및 이차 아미노산의 감도 높은 형광 유도체를 얻었으며, AQC 전컬럼법을 사용하여 형광검출로 닭의 조직 및 혈액시료 내 아미노산을 분석하였다. AQC법을 사용하는 새로운 HPLC법을 개발하여 생체 시료 낸 호모시스틴(Hcy)과 시스타티오닌(Cysta)의 농도를 측정하였다. 닭의 간, 가슴살 및 혈액 시료 내 Hcy의 평균 총 농도는 각각 9.66, 4.66 및 7.77 nmol/g 이었으며, Cysta의 평균 농도는 각각 15.93, 2.55 및 8.08 nmol/g이었다. AQC법은 생체시료 내 아미노산, Hcy 및 Cysta의 농도를 빠르고 쉽게 측정하는데 사용할 수 있다.

The AQC has been used for the derivatization of primary and secondary amino acids to yield fluorescent derivatives with high sensitivity. The AQC precolumn method was applied to analyze the amino acids using florescent detector for tissue and blood samples of chickens. A new HPLC method was developed to measure the concentrations of homocysteine (Hcy) and cystathionine (Cysta) in biological samples by using AQC method. The averaged total concentrations of Hcy were obtained as 9.66, 4.66, and 7.77 nmol/g in liver, breast and blood samples of chicken, respectively. Also, the averaged values of Cysta concentrations were obtained as 15.93, 2.55, and 8.08 nmol/g in liver, breast, and blood samples, respectively. The AQC method was rapid and easy to measure the concentrations of amino acids, Hcy and Cysta in biological samples.

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