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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 46, Number 4
JKCSEZ 46(4)
August 20, 2002 

 
Title
Simultaneous Determination of Tryptophan and Tyrosine by Spectrofluorimetry Using Multivariate Calibration Method

다변량 분석법을 이용한 Tryptophan과 Tyrosine의 형광분광법적 정량
Author
Sang Hak Lee, Ju Eun Park, Bum Mok Son

이상학,박주은, 손범목
Keywords
Abstract
형광분광법에 의하여 주성분 회귀분석(principal component regression, PCR)과 부분 최소자승법(Partial least squares, PLS)을 이용하여 아미노산(Tryptophan and Tyrosine)을 동시에 정량하는 방법에 대하여 연구하였다. 아미노산 혼합물의 형광 스펙트럼은 들뜸파장을 257nm로 고정하여 측정하였다. 두 가지 아미노산이 서로 다른 농도로 혼합되어 있는 32개의 시료용액을 280nm∼500nm 범위에서 스펙트럼들을 얻었고 이를 이용하여 PCR과 PLS회귀모델을 얻었다. 두 가지 아미노산이 서로 다른 농도로 포함된 6개의 외부검정용 시료들의 스펙트럼들을 이용해서 회귀모델의 적합성을 검정하기 위하여 외부검정용 시료의 농도를 계산하였다. 계산된 농도를 이용하여 relative standard error of prediction(RSEPa)를 얻었고 같은 방법으로 overall relative standard error of prediction(RSEPm) 도 구하였다

A spectrofluorimetric method for the simultaneous determination of amino acids (tryptophan and tyrosine) based on the application of multivariate calibration method such as principal component regression and partial least squares (PLS) to luminescence measurements has been studied. Emission spectra of synthetic mixtures of two amino acids were obtained at excitation wavelength of 257 ㎚. The calibration model in PCR and PLS was obtained from the spectral data in the range of 280-500 ㎚ for each standard of a calibration set of 32 standards, each containing different amounts of two amino acids. The relative standard error of prediction (RSEPa) was obtained to assess the model goodness in quantifying each analyte in a validation set. The overall relative standard error of prediction (RSEPm) for the mixture obtained from the results of a validation set, formed by 6 independent mixtures was also used to validate the present method.

Page
309 - 316
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