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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 12, Number 5
BKCSDE 12(5)
May 20, 1991

Title	Study on the Biosynthesis on Neomycin: Characterization of Isocitrate Dehydrogenase of the Neomycin Producer, Streptomyces fradiae and its Possible Relation to the Regulation of Biosynthesis of Neomycin
Author	Chang Hoon Lee, Yang Mo Goo*, Kong Hwan Kim
Keywords
Abstract	S. fradiae showed very high activity of isocitrate dehydrogenase compared to other microorganisms. The activity of this enzyme was increased with the growth of the organism. But the increase might not imply its involvement in the growth. Rather its increased activity seemed to have a connection with the biosynthesis of neomycin. The enzyme showed high specificity toward NADP+ and D-isocitrate with Km values of 5.75 and 6.74 μM, respectively, It was activated by Mn2+. Its molecular weight was estimated from its gel retardation coefficient to be in the range of 61,000-63,000 daltons and its optimum pH was 8.0. The enzyme was thermally unstable.
Page	504 - 509
Full Text	PDF

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