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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 35, Number 10
BKCSDE 35(10)
October 20, 2014 

Comprehensive Proteome Analysis of the Excretory/Secretory Proteins of Toxoplasma gondii
Won-Kyu Lee, Hye-Jin Ahn, Je-Hyun Baek, Chong-Heon Lee, Yeon Gyu Yu*, Ho-Woo Nam*
Excretory/secretory protein, Toxoplasma gondii, Micronemal protein, Rhoptry protein, Dense granule protein
Proteomic analyses of the excretory/secretory proteins from the RH strain of Toxoplasma gondii have been performed to understand their functions in the host-parasite interaction. A total of 34 proteins were identified from LC/MS/MS analysis and their abundance was estimated by spectral counting methods. Among them, 8 species of micronemal proteins (MICs), 2 species of rhoptry proteins (ROPs), and 6 species of dense granular proteins (GRAs) were confirmed. Besides these, 18 species of protein were newly identified, and their cellular functions were estimated from sequence analysis. The three most abundant of the 34 identified extractor/ secretory proteins−GRA1, GRA7 and GRA2−were confirmed to be highly expressed in T. gondii using the spectral count method. This phenomenon is another demonstration of the importance of GRA proteins for the penetration and survival of T. gondii.
3071 - 3076
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