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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 32, Number 9
BKCSDE 32(9)
September 20, 2011 

Emission Detection of Mercuric Ions in Aqueous Media Based-on Dehybridization of DNA Duplexes
Byul Nim Oh, Qiong Wu, Mi Sun Cha, Hee Kyung Kang, Jin Ah Kim, Ka Young Kim, Eswaran Rajkumar, Jinheung Kim *
Metal-to-ligand transfer emission, DNA intercalation, Poly(dT), Mercuric ion sensing, Light switch Ru compound, Mismatch detection
To quantify the presence of mercuric ions in aqueous solution, double-stranded DNA (dsDNA) of poly(dT) was employed using a light switch compound, Ru(phen)2(dppz)2+ (1) which is reported to intercalate into dsDNA of a right-handed B-form. Addition of mercuric ions induced the dehybridization of poly(dT)·poly(dA) duplexes to form a hairpin structure of poly(dT) at room temperature and the metal-to-ligand charge transfer emission derived from the intercalation of 1 was reduced due to the dehybridization of dsDNA. As the concentration of Hg2+ was increased, the emission of 1 progressively decreased. This label-free emission method had a detection limit of 0.2 nM. Other metal ions, such as K+, Ag+, Ca2+, Mg2+, Zn2+, Mn2+, Co2+, Ni2+, Cu2+, Cd2+, Cr3+, Fe3+, had no significant effect on reducing emission. This emission method can differentiate matched and mismatched poly(dT) sequences based on the emission intensity of dsDNA.
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