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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 32, Number 1
BKCSDE 32(1)
January 20, 2011 

 
Title
31P NMR Spectroscopy Revealed Adenylate kinase-like Activity and Phosphotransferase-like Activity from F1-ATPase of Escherichia coli
Author
Hyun Won Kim
Keywords
31P NMR, F1-ATPase, Escherichia coli, Adenylate kinase-like activity, Phosphotransferase-like activity
Abstract
Adenylate kinase-like activity and phosphotransferase-like activity from F1-ATPase of Escherichia coli was revealed by 31P NMR spectroscopy. Incubation of F1-ATPase with ADP in the presence of Mg2+ shows the appearance of 31P resonances from AMP and Pi, suggesting generation of AMP and ATP by adenylate kinase-like activity and the subsequent hydrolysis to Pi. Incubation of F1-ATPase with ADP in the presence of methanol shows additional peak from methyl phosphate, suggesting phosphotransferase-like activity of F1-ATPase. Both adenylate kinase-like activity and phosphotransferase-like activity has not been reported from F1-ATPase of Escherichia coli. 31P NMR could be a valuable tool for the investigation of phosphorous related enzyme.
Page
183 - 185
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