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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 32, Number 1
BKCSDE 32(1)
January 20, 2011

Title	³¹P NMR Spectroscopy Revealed Adenylate kinase-like Activity and Phosphotransferase-like Activity from F1-ATPase of Escherichia coli
Author	Hyun Won Kim
Keywords	³¹P NMR, F₁-ATPase, Escherichia coli, Adenylate kinase-like activity, Phosphotransferase-like activity
Abstract	Adenylate kinase-like activity and phosphotransferase-like activity from F₁-ATPase of Escherichia coli was revealed by ³¹P NMR spectroscopy. Incubation of F₁-ATPase with ADP in the presence of Mg²⁺ shows the appearance of ³¹P resonances from AMP and Pi, suggesting generation of AMP and ATP by adenylate kinase-like activity and the subsequent hydrolysis to Pi. Incubation of F₁-ATPase with ADP in the presence of methanol shows additional peak from methyl phosphate, suggesting phosphotransferase-like activity of F₁-ATPase. Both adenylate kinase-like activity and phosphotransferase-like activity has not been reported from F₁-ATPase of Escherichia coli. ³¹P NMR could be a valuable tool for the investigation of phosphorous related enzyme.
Page	183 - 185
Full Text	PDF

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