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ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 30, Number 9
BKCSDE 30(9)
September 20, 2009 |
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Title |
Interaction of ct-DNA with 2,4-Dihydroxy Salophen
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Author |
Mohammad Reza Azani, Azin Hassanpour, Abdol Khalegh Bordbar*, Valiollah Mirkhani |
Keywords |
DNA, Schiff base, Interaction, Fluorescence, UV-Vis
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Abstract |
In the present study, at first, 2,4-Dihydroxy Salophen (2,4-DHS), has been synthesized by combination of 1, 2-diaminobenzene and 2,4-dihydroxybenzaldehyde in a solvent system. This ligand containing meta-quinone functional groups were characterized using UV-Vis and IR spectroscopies. Subsequently, the interaction between native calf thymus deoxyribonucleic acid (ct-DNA) and 2,4-DHS, was investigated in 10 mM Tris/HCl buffer solution, pH 7.2, using UV-visible absorption and fluorescence spectroscopies, thermal denaturation technique and viscosity measurements. From spectrophotometric titration experiments, the binding constant of 2,4-DHS with ct-DNA was found to be (1.1 ± 0.2) × 104 M?1. The fluorescence study represents the quenching effect of 2,4-DHS on bound ethidium bromide to DNA. The quenching process obeys linear Stern-Volmer equation in extended range of 2,4-DHS concentration. Thermal denaturation experiments represent the increasing of melting temperature of DNA (about 3.5 oC) due to binding of 2,4-DHS. These results are consistent with a binding mode dominated by interactions with the groove of ct-DNA.
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1973 - 1977 |
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