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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 27, Number 7
BKCSDE 27(7)
July 20, 2006

Title	Purification and Characterization of Substance P-related Peptide from the Body of the African Lungfish, Protopterus dolloi
Author	Chan-Hee Kim, Eun Jung Kim, Hye-Jin Go, Hyung Ho Lee, Yong-Ki Hong, Hyung-Rak Kim, Joon Ki Chung, Jang-Su Park, Yojiro Muneoka, Nam Gyu Park*
Keywords	African lungfish, Purification, Substance P
Abstract	The peptide with structural similarity to mammalian substance P (M-SP) has been isolated from extract of the body of the African lungfish, Protopterus dolloi, using the rectum of the newt as the bioassay system. The primary structure of the SP-related peptide was identified as Lys-Pro-Arg-Pro-Asp-Gln-Phe-Tyr-Gly-Leu-Met-NH₂ (L-SP) and contained four substitutions (Lys¹ → Arg, Arg³ → Lys, Asp⁵ → Gln, and Tyr⁸ → Phe) compared with M-SP; this structure is identical to that of the peptide isolated from the gut of the Australian lungfish. Circular dichroism spectra showed that L-SP had an unordered structure in the buffer solution and phospholipid bilayers. This peptide was found to have an excitatory effect on rectal muscle tissues of newt, quail, and fish. L-SP also had a more potent vasodilatory effect on the guinea-pig aorta than that of M-SP. The identification of the peptide provides evidence that SP family, hitherto confined to mammals, have a widespread occurrence in lungfish.
Page	1015 - 1019
Full Text	PDF

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