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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 26, Number 3
BKCSDE 26(3)
March 20, 2005

Title	Fluorescence Quenching of Green Fluorescent Protein during Denaturation by Guanidine
Author	Kichul Jung, Jaebok Park, Pil-Jae Maeng, Hackjin Kim*
Keywords	GFP, Fluorescence, Denaturation, Quencher
Abstract	Fluorescence of green fluorescent protein mutant, 2-5 GFP is observed during denaturation by guanidine. The fluorescence intensity decreases exponentially but the fluorescence lifetime does not change during denaturation. The fluorescence lifetime of the denatured protein is shorter than that of native form. As the protein structure is modified by guanidine, solvent water molecules penetrate into the protein barrel and protonate the chromophore to quench fluorescence. Most fluorescence quenchers do not affect the fluorescence of native form but accelerate the fluorescence intensity decay during denaturation. Based on the observations, a simple model is suggested for the structural change of the protein molecule during denaturation.
Page	413 - 417
Full Text	PDF

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