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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 21, Number 10
BKCSDE 21(10)
October 20, 2000 

Binding Mode of [Ruthenium(II)( 1, 10-Phenanthroline)2L2+ to Poly(dG)·poly( dC)·poly(dC)+ Triplex DNA
Chang-Beom Cho, Tae-Sub Cho, Seog K. Kim, Bok-Jo Kim, Sung Wook Han, Maeng-Joon Jung
Binding geometries of [Ru(Ⅱ)(1,10-phenanthroline)₂L]²+ complexes (where L = dipyrido [3,2-a:2’,3’-c]phena-zine (DPPZ) or benzodipyrido[3,2-a:2’,3’-c] phenazine (BDPPZ)) to poly(dG)·poly(dC)·poly(dC)+ triplex DNA (CGC+ triplex) has been investigated by linear dichroism and normal absorption spectroscopy. Analysis of the linear dichroism for the CGC+ triplex and [Ru(Ⅱ)(phen)₂BDPPZ]²+ complex indicates that the extended ligand of the metal complex lie perpendicular to the polynucleotide helix axis. Together with strong hypochromism and red shift in the interligand absorption region, we concluded that the extended BDPPZ or DPPZ ligand in-tercalated between the bases of polynucleotide. The spectral properties of the metal complexes bound to CGC+ triplex are similar to those bound to poly(dA)[poly(dT)]² triplex (Choi et al., Biochemistry 1997, 36, 214), sug-gesting that the metal complex is located in the minor groove of the CGC+ triplex.
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