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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 31, Number 2
JKCSEZ 31(2)
April 20, 1987

Title	Liquid Chromatography of Non-Chromophores by Indirectly Photometric Detection 간접 분광광도 측정에 의한 비발색단의 액체크로마토그래피
Author	Sam-Woo Kang, Hae-Beom Oh, Young-Ja Moon 강삼우, 오해범, 문영자
Keywords
Abstract	역상 액체크로마토그래피에 의한 지방족 알코올, 케톤 및 에스테르를 분리하는데 검출 시약으로 brilliant green 에 관하여 연구하였다. 발색단을 가지고 있지 않은 이들 시료들을 brilliant green을 사용함으로써 가시영역인 625nm에서 분광광도 검출기로서 간접적으로 검출할 수 있었다. 또한 몇가지 혼합시료들을 좋은 분리도와 비교적 높은 감도로서 분리 및 검출할 수 있었다. Brilliant green was examined as a detection reagent in the separation of some aliphatic alcohols, ketones and esters by the reverse-phase liquid chromatography. These samples not possessing chromophore could be indirectly detected by photometric detector with the assistance of brilliant green in visible range (625nm). Some mixtures of samples were also able to separated and detected with good base line resolution and comparatively high sensitivity.
Page	184 - 189
Full Text	PDF

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