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  • 09월 12일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제110회 대한화학회 학술발표회, 총회 및 기기전시회 Buforin IIb as a peptide vector for the nonviral delivery of nucleic acids

2012년 9월 9일 11시 50분 02초
BIO.P-764 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 17일 (수요일) 16:00~19:00
저자 및
김지수, 하상수
경희대학교 화학과, Korea
RNA interference (RNAi) is a natural response of eukaryotic cells to double-stranded RNA (dsRNA) leading to silencing of homologous gene transcripts by ~21 nucleotide (nt) dsRNAs, termed short interfering RNAs (siRNAs). While siRNAs have been rapidly appreciated to silence genes, efficient and non-toxic vectors for primary cells and for systemic in vivo delivery remain lacking. Several siRNA-delivery vehicles, including cell-penetrating peptides (CPPs), have been developed but their utility is often restricted by entrapment following endocytosis. Hence, developing CPPs that promote endosomal escape is a prerequisite for successful siRNA implementation. We here present buforin IIb, a synthetic analog of buforin II that contains a proline hinge between the two α-helices and a model α-helical sequence at the C-terminus (3× RLLR), in order to facilitate endosomal release of siRNAs. Stable buforin IIb-conjugated siRNAs enter entire cell populations and rapidly promote endosomal escape, resulting in robust RNAi responses in MCF-7 cells, with buforin IIb-mediated delivery being independent of cell confluence. These results imply that the peptide, in addition to having utility for RNAi screens in vitro, displays great therapeutic potential.