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Fluorescence lifetime imaging microscopy for screening amyloid aggregation inhibitors

등록일
2005년 8월 10일 16시 07분 32초
접수번호
0473
발표코드
22P101포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅱ>
발표형식
포스터
발표분야
물리화학
저자 및
공동저자
고정민, 이민영1
이화여자대학교 나노과학부,
1이화여자대학교 화학과/나노과학부,
Alzheimer’s disease(AD) is caused by amyloid aggregation in neuron. There have been tremendous efforts to explore a new efficient inhibitor for amyloid aggregates as a curing agent of AD. In this work, we have used fluorescence lifetime imaging microscopy (FLIM) for screening amyloid aggregation inhibitors. First, we designed a new FRET amyloid aggregation probe which consists of Alexa 488 for an energy donor, amyloid11-25 and azulene for an energy acceptor. The amyloid conformation probe exhibits different fluorescence lifetimes as in monomers and in micellar structures. The amyloid micellar structures were prepared on a cover glass and the probe lifetime distribution was mapped by FLIM. The inhibitor screening was accomplished by monitoring the FLIM images and bimodal fluorescence lifetime distributions. It appeared that our method is much more powerful than the conventional Th T binding assay.