Current time in Korea 14:31 Aug 23 (Fri) Year 2019 KCS KCS Publications
KCS Publications
My Journal Log In Register
HOME > Search > Browsing(JKCS) > Archives

Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 43, Number 4
JKCSEZ 43(4)
August 20, 1999 

 
Title
Spectrofluorimetric Determination of Fe(III) with 4,5-Dihydroxy-1,3-Benzenedisulfonic Acid

4,5-Dihydroxy-1,3-Benzenedisulfonic Acid를 이용한 Fe(III)의 분광형광법 정량
Author
Hye-Seon Kim, Hee-Seon Choi

김혜선, 최희선
Keywords
Abstract
형광체로서 4,5-dihydroxy-1,3-benzenedisulfonic acid (Tiron)를 이용하여수용액속의 Fe(III)를 정량할 수 있는 분광형광법에 대해 연구하였다. 물에 잘 용해되는 Tiron은 좋은 형광시약이지만, Fe(III)와 착물을 이루면 소광 효과로 인해 형광의 세기가 Fe(III)의 농도에 비례하여 감소하였다 pH 4.5에서 Fe(III)에 의해 소광 효과를 보여주는 tiron의 들뜸 및 형광 파장은 각각 312 nm와 341nm 이었다. 감도는 Tirondml의 농도가 1.0×10-2M일 때 가장 좋았다. 소광효과를 증가시키기 위여 Fe(III)-Tiron 착물 용액을 80℃에서 9O분 동안 가열하였다. Fe(III)의 경우 가장 방해하는 이온은 Cu(II) 이었는데, 이는 pH를 조절하거나 EDTA를 넣어 주므로서 방해 효과를 없앨 수 있었다.Fe(III)의 직선 농도범위는 5.0×10-7M 에서 6.0×10-5M까지 이었다. 이 제시된 방법의 검출한계는 2.8×10-6M이었고, 합성시료에서 Fe(III)의 회수는 거의 정량적으로 이루어졌다. 실험결과를 종합하여 보면, 이 제시된 방법을 이용하면 실제 시료에 들어있는 Fe(III)을 정량할 수 있을 것이다.

A spectrofluorimetric method for the determination of Fe(IIl) in aqueous solution with 4,5-dihydroxy-1,3-benzenedisulfonic acid(Tiron) as a fluorimetric reporter was developed. Tiron, which is very soluble in water,is a good fluorimentric reagent However, when Tiron was complexed with Fe(III), the fluorescent intensity was decreased proportionally with the concentration of Fe(III) by a quenching effect. The excitation and fluorescene wavelength of Tiron showing the quenching effect by Fe(III) at pH 4.5 were 312nm and 341nm, respectively. The highest sensitivities were shown at Tiron concentration of l.0 × 10-2M. To enhance the quenching effect, the Fe(III)-Tiron complex solution was heated to 80℃ for 90 minutes. As for Fe(III), the most interfering ion was Cu(II). The interference effects could be mostly eliminated by pH adjustment or by adding EDTA. The concentration ranges showing the linear response to Fe(III) was from 5.0× 10-7M to 6.0 × 10-5M With this proposed method, the detection limits of Fe(III) was 2.8 × 10-6M. Recovery of Fe(lII) in a synthetic sample was almost quantitative. Based on experimental results,lt is proposed that the above technique can be applied to the practical determination of Fe(III).

Page
423 - 429
Full Text
PDF