Current time in Korea 12:14 Oct 24 (Sat) Year 2020 KCS KCS Publications
KCS Publications
My Journal  Log In  Register
HOME > Search > Browsing(JKCS) > Archives

Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 42, Number 1
JKCSEZ 42(1)
February 20, 1998 

A Large Scale Separation & Purification of Cyanobacterial Toxins

남조류 독성물질의 대량분리 및 정제
Sukchang Yoon, Keunyoung Park, Dongjin Pyo*

윤석창, 박권영, 표동진
남조류 독성물질인 Microcystins은 자연계에 매우 미량 존재하기 때문에 이를 분리 및 정제하기가 쉽지 않다. 본 연구에서는 자연계에 존재하는 남조류 동결건조시료로부터 Microcystins RR과 LR을 분리 및 정제하는 새로운 방법을 개발하였다. 약 7.5g의 실리카겔을 정지상으로 사용하고 혼합용액인 ethyl acetate: iso-propanol:water(30: 45: 25)을 이동상으로 사용하는 open column system으로 시료를 전개한 후 용액을 3ml간격으로 받아냈다. 받아낸 용액중의 Microcystins RR과LR은 TLC와 HPLC(high performance Liquid Chromatography)를 이용하여 확인한 후 농축, 정제하였다.

It is very difficult to separate and purify the microcystins, cyanobacterial toxins since it exist in a trace level in natural lakes. In this paper, we developed a new analytical method to separate and purify the microcystin RR and LR from the freeze-dried cyanobacterial cells in natural lakes. We used 7.5 g silica gel as a stationary phase and ethyl acetate: isopropanol: water (30: 45: 25) as a mobile phase and microcystins were eluted using an open column. The eluting solvent was collected in a small bottle at the intervals of 3 mL and the fractions were chromatographed with HPLC to confirm the microcystin RR and LR.

51 - 56
Full Text