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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 41, Number 12
JKCSEZ 41(12)
December 20, 1997

Title	Solubilization of Brain Phospholipase D by Taurodeoxycholate: Activational Effect of Some Matal Ions Taurodeoxycholate에 의한 뇌 포스포리파제 D의 용해:몇 금속이온의 활성화 효과
Author	Seok-Woo Choi, Myung-Un Choi* 최석우,최명운
Keywords
Abstract	쥐의 미크로좀 포스포리파제 D(PLD)를 센 이온세기 상태에서 0.2% taurodeoxycholate를 사용하여용해시켰다 포스포리파제 D의 활성은 기질로 방사성 동위원소로 표지된 dipalmitoylphosphatidylcholine을 사용하여 생성된 phosphatidic acid(PA)를 측정하여 결정하였다. 용해된 PLD의 최적 pH와 온도는 각각 6.5와 30。C로서 용해되기 전 미크로좀 상태의 PLD와 비슷하였다. 올레산의 활성화 효과는 4 mM 농도에서 관찰되었다. PLD 활성도에 미치는 금속이온 영향을 조사한 결과 Mg2+, Ca2+, Sr2+, Ba2+와 같은 알칼리 토금속은 모두 PA 생성을 촉진시킨 반면, Cu2+, Cd2+, Al3+, Ni2+, V5+는 억제하였다. Microsomal phospholipase D (PLD) in rat brain was solubilized employing 0.2 % taurodeoxycholate in high ionic strength. Phopholipase D activity was determined by measuring product phophatidic acid (PA) using isotope-labelled dipalmitoylphophatidylcholine as a substrate. The solubilized PLD showed an optimal pH of 6.5 and the highest activity at 30 ℃. These properties were similar to those of microsomal PLD before solubilization. The stimulatory effect of oleic acid was observed at the concentration of 4 mM. When effects of metal ions on PLD activity were examined, alkaline earth metals such as Mg2+, Ca2+, Sr2+, Ba2+ promoted the PA production but Cu2+, Cd2+, Al3+, Ni2+, V5+ showed inhibitory effects.
Page	672 - 676
Full Text	PDF

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