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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 41, Number 7
JKCSEZ 41(7)
February 20, 1997 

 
Title
Chicken Liver Tissue-Based Amperometric Biosensor for the Determination of Hydrogen Peroxide

닭의 간조직을 이용한 과산화수소 정량 바이오센서
Author
Kil-Joong Yoon, Sang-Yong Pyun, Hyo-Shik Kwon

윤길중,변상용,권효식
Keywords
Abstract
과산화수소의 정량을 위하여 닭의 간조직과 ferrocene을 탄소반죽에 혼입시켜 만든 전류법 바이오센서의 감응 특성이 조사되었다. 또한 조사된 전극 퍼텐샬 범위(-05-+0.O5 V vs. Ag/AgCI) 내에서 t96%=13sec의 빠른 감응시간과 5.1×10-5M(S/N=3)의 검출한계를 보여 주었으며, 감응에 방해가 된다고 여겨지는 물질들에 대하여 좋은 선택성을 보여 주었다. 생촉매 안정성이 좋았으며, 이동매체를 사용하지 않는 전극의 제조가능성을 제시하였다.

The response characteristics of a new biosensor, which was developed by co-immobilisation of chicken liver tissue and ferrocene in a carbon paste matrix for the amperometric determination of hydrogen peroxide, was evaluated. In the range of electrode potential observed (-O.5∼ +0.05 V vs. Ag/AgCl), the bioelectrode showed the response time (t95%) as low as 13 set and the detection limit of 5.1x10-5 M, and exhibited a good selectivity in the case of the addition of the possible interferents tested. Also it offered a high biocatalytic stability and opened up the possibility for the construction of a mediatorless biosensor.

Page
343 - 350
Full Text
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