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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 41, Number 4
JKCSEZ 41(4)
August 20, 1997 

Urinary Profiling of Endogeneous Estrogens Using GC/MS

GC/MS에 의한 뇨 중 내인성 에스트로겐의 Profiling
Seon Hwa Lee, Yoon Jung Yang, Taewook Kim, Ki-Jung Paeng, Bong Chul Chung

성 호르몬으로서의 역할과 암 성장의 잠재적 저해 효과를 지니고 있는 에스트로겐의 대사체들을 동시에 분석하기 위하여 Serdolit AD-2 수지와 효소 가수분해, 액체-액체 추출방법을 이용한 전처리 과정을 확립하였으며, MSTRA/TMSCI 혼합액에 의해 trimethylsilylether 형태로 유도체화된 에스트로겐들을 GC/MS의 SIM(selected ion monitoring) 방법으로 동시에 분석, 정량할 수 있는 조건을 설정하였다. 그 결과 회수율은 80.97∼97.81%이었고, within-a-day 및 day-to-day 분석에서의 RSD값은 각각 0.24∼25.35%, 1.05∼23.94%이었으며, 이 방법으로 소변 내에 존재하는 19종의 에서ㅡ트로겐 대사체들을 정량하여 한국인 정상인 참고치(여자:22∼35세, 남자:39∼57세)를 설정하였다.

Estrogens, which play an important role in sex hormone and potential inhibitor of cancer cell proliferation were profiled for normal female and male. The nineteen endogeneous estrogens were simultaneously analyzed by selected ion monitoring method of GC/MS. Urinary estrogens were extracted by using Serdolit AD-2 resin, hydrolyzed with β-glucuronidase/arylsulfatase from Helix pomatia, liquid-liquid extraction and quantitatively derivatized by MSTFA/TMSCl mixture in order to be detected on the GC/MS. The good quality-control data were obtained through the precision and accuracy test and the recovery range of them was 80.97∼97.81%. The Korean reference values of urinary estrogens were established and differences were found in normal female compared with normal male.

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