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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 38, Number 10
JKCSEZ 38(10)
August 20, 1994 

Development of New Analysis Method of Cyanobacterial Toxins in Reservoirs

호수에서의 남조류 독성물질의 새로운 분석법 개발
Dongjin Pyo, Kiseob Song, Sukchang Yoon, Bomchul Kim, Dai Woon Lee
남조류의 독성물질인 microcystin을 HPLC로 분석하기 위해 지금까지는 ODS cartridge를 사용하였으나 본 연구에서는 CN cartridge를 사용하는 새로운 분석 방법을 시도하였다. CN cartridge는 0.5M 초산 용액으로 처리되었으며 Microcystins RR과 LR을 용리시키기 위해 30% Acetonitrile 용액이 사용되었다. CN cartridge를 이용하는 분석 방법이 기존의 ODS cartridge를 이용하는 방법보다 훨씬 더 정확한 정량을 할 수 있었다. 특히 Microcystin LR의 경우 피크 면적에서 현저한 차이를 보였다.

A new HPLC method for the analysis of cyanobacterial toxins, i.e. microcystin was developed using cyano-type prepacked cartridge while the conventional method was to utilize ODS cartridge. The cartridge was washed with 0.5 M acetic acid, then microcystins RR and LR were eluted from the cartridge with 30% acetonitrile. A better degree of quantitation was observed than with a ODS cartridge. Especially, in the case of microcystin LR a great difference in peak area was observed.

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