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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 37, Number 11
JKCSEZ 37(11)
October 20, 1993 

 
Title
Amperometric Enzyme Electrode for the Determination of NH4+

NH4+정량을 위한 Amperometric Enzyme Electrode
Author
Moo Lyong Seo, Jae Sang Kim, Shim Sung Lee, Zun Ung Bae, Heung Lark Lee, Tae Myung Park

서무룡, 김재상, 이심성, 배준웅, 이흥락, 박태명
Keywords
Abstract
Immobilon-AV Affinity membrane에 L-glutamate dehydrogenase를 고정하여 유리질 탄소전극에 부착시킨 전극을 사용하여 암모니아를 전류법으로 정량하였다. 이때 환원형의 NADH가 NAD+로 산화될 때 전류를 +1.0 volt vs. Ag/AgCl에서 측정하였다. 효소 고정화된 membrane을 부착시킨 전극의 감응 특성은 다음과 같다. 곧 직선 감응 농도 범위는 4.0 × 10-5 to 4.0 × 10-4 M이었으며, 정량한계는 2.0 × 10-6 M이었다. 또한 감응 시간은 2분이었으며 효소 고정화된 막의 최적 pH와 수명은 각각 pH 7.3∼7.6 (Dulbecco's buffer 용액)과 25일이었다. 그리고 다른 생리활성 물질의 방해는 없었다.

Enzyme electrodes for amperometric measurement of ammonia was prepared by immobilization of L-glutamate dehydrogenase on an Immobilon-AV Affinity membrane and attachment to a glassy carbon electrode. Reduced nicotinamide adenine dinucleotide (NADH) was used as the electroactive species. The electrochemical oxidation of NADH was monitored at +1.0 volt vs. Ag/AgCl. Response was linear from 4.0 × 10-5 to 4.0 × 10-4 M. The detection limit was 2.0 × 10-6 M. Response time, the optimum pH and life time of enzyme immobilized membrane were 2 min, pH 7.3∼7.6 (Dulbecco's buffer solution) and about 25 days respectively. When the enzyme electrode was applied to the NH4+ determination with amperometric method, other physiological materials had no interference.

Page
937 - 942
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