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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 36, Number 5
JKCSEZ 36(5)
October 20, 1992 

 
Title
The Development of Arginine-Selective Membrane Electrode Using Tissue Slices of the Rose of Sharon

무궁화꽃을 이용한 L-Arginine Sensor 개발에 관한 연구
Author
Ui-Rak Kim, Ki-Jae Nam, Sung-Moon Choi

김의락, 남기재, 최성문
Keywords
Abstract
흰 무궁화꽃의 씨방을 이용하여 Tissue electrode를 제조하고, L-arginine을 정량하기 위한 최적조건을 조사한 결과 0.1M phosphate 완충용액에서 pH 8.0, 온도 25℃, 조직두께 20 μm이었을 때이며, 이에 정량 가능한 직선범위는 5.0 × 10-3M ∼ 1.0 × 10-1M이었으며, 감응도는 54.0 mV/decade로 나타났고, 감응시간은 7∼8분이 소요되었다. Km값은 6.4 × 10-6(M)이다.

It is shown that receptacle tissue of the rose of sharon can serve as an effective biocatalyst by the construction of a potentiometric membrane electrode with response and selectivity for arginine. Electrodes using this tissue in conjunction with NH3 gas sensing probes yield good response to arginine in 10-1M to 10-3M range and retain activity for a least 1 weeks. The arginine-selective membrane electrode using tissue slices of the rose of sharon have good selectivity in presence of other amino acids. The optimum conditions are pH 8.0, 25℃ and 20 μm thickness of slices in 0.1 M phosphate buffer solution and Km values is 6.4 × 10-6(M).

Page
753 - 759
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