ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 36, Number 5
JKCSEZ 36(5)
October 20, 1992 

It is shown that receptacle tissue of the rose of sharon can serve as an effective biocatalyst by the construction of a potentiometric membrane electrode with response and selectivity for arginine. Electrodes using this tissue in conjunction with NH3 gas sensing probes yield good response to arginine in 10^-1M to 10^-3M range and retain activity for a least 1 weeks. The arginine-selective membrane electrode using tissue slices of the rose of sharon have good selectivity in presence of other amino acids. The optimum conditions are pH 8.0, 25℃ and 20 μm thickness of slices in 0.1 M phosphate buffer solution and Km values is 6.4 × 10^-6(M).