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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 36, Number 4
JKCSEZ 36(4)
August 20, 1992 

 
Title
Metachromasy of Methylene Blue on the Bacteriorhodopsin Incorporated into L-α-lecithin Vesicle

L-α-lecithin으로 재구성된 Bacteriorhodopsin Vesicle에서 Methylene Blue의 Metachromasy
Author
Hong Lee, Huyn-Ock Pae, Chun-Ock Lim, Hoo-Seol Lee

이홍, 배현옥, 임춘옥, 이후설
Keywords
Abstract
L-α-lecithin vesicle, bacteriorhodopsin(BR) 그리고 인지질로 재구성된 BR vesicle(InBR vesicle)의 각각의 수용액에서 methylene blue(MB)의 흡수성질을 20℃에서 60℃까지 흡수분광법으로 측정하였다. 인지질 vesicle에서 묽은 농도의 MB는 단위체와 이합체 사이에 평형을 이루었고, 고농도의 MB는 oligomer를 형성하였다. 대부분의 경우에 지질(vesicle)의 농도가 매우 높아지면 점점 단위체로 재분배 되었다. 일정한 MB 농도에 BR를 첨가할 때 흡광도비(α/β)는 감소되며 oligomer가 형성되었다. MB는 InBR vesicle의 상전이 온도 부근에서 흡광도비(α/β)가 증가했으나 인지질 vesicle에서는 상전이에 무관했다. 이것은 InBR vesicle 표면 위에서 회합하는 MB 분자들은 지질의 상전이에 영향을 받아 단위체로 재분배되는 것으로 사료된다.

Absorptioon properties of methylene blue (MB) in L-α-lecithin vesicle, bacteriorhodopsin and incorporated bacteriorhodopsin (InBR) vesicle systems at 20∼60℃ has been studied by adsorption spectroscopy. The equilibrium of MB between monomer and dimer in lecithin vesicles has been existed at low concentration of MB, but oligomer has been formed in vesicle at higher concentration of MB. In most cases, the MB cluster was redistributed to monomer at the concentration of lecithin vesicles. Adding BR to constanr concentration of MB deceased the adsorption ratio (α/β) of MB, and MB was formed oligomeric aggregate. abosorption ratio (α/β) of MB was increased during phase transition of InBR vesicles, but independent of phase transition of lecithin vesicles. It suggested that aggregate of MB on the surfaces of InBR vesicles were redistributed to monomer under the influence of lipid phase transition.

Page
504 - 510
Full Text
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