ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 36, Number 1
JKCSEZ 36(1)
February 20, 1992 

The method for investigating the retention behavior and separation optimization was studied to develop a reversed-phase liquid chromatography of eleven thiocarbamates that are used as herbicides. As an isocratic elution system, the statistical simplex technique was applied to find the optimum separation conditions. The resolution was quantitatively evaluated by using COF and ORM method. For thiocarbamate herbicides in which the elution order varies dramatically with solvent composition, the ORM method gave better result than the COF method. For the solvent composition of mobile phase in the ORM method, the ratio of methanol : acetonitrile : tetrahydrofuran : water was 16 : 29 : 2 : 53. Also in this research, an adjusted COF (COF) method was proposed to rectify the defect in the current COF method, and the compositional ratio of mobile phase containing methanol : acetonitrile : water was 29.5 : 21.5 : 49.0 result of which is similar to that obtained by the ORM method.