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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 34, Number 5
JKCSEZ 34(5)
October 20, 1990 

 
Title
Preparation of the Citrobacter freundii Bio-Sensor for the Determination of Glucose and Its Applications

Glucose 정량을 위한 Citrobacter freundii Bio-Sensor의 개발과 그 응용
Author
Gwon-Shik Ihn, Young-Seuk Hong, Ui-Rak Kim, Seh-Yong Jang, Moo-Jeong Sohn

인권식, 홍영석, 김선락, 장세용, 손무정
Keywords
Abstract
Citrobacter freundii와 그의 organelle을 CO2 gas-sensor에 고정시켜서 glucose 정량을 위한 biosensor를 조립하였다. 박테리아의 경우가 organelle 보다 감응은 우수하였으나 감응시간은 organelle의 경우가 훨씬 짧았다. 박테리아 전극은 30℃의 온도에서 pH 7.0 인 0.2M tris-HCl 완충용액을 사용하면 7.0 × 10-4 - 1.0 × 10-2 M glucose 농도범위에서 42.2 mV/decade의 감응기울기를 나타냈다. 이 전극은 glucose 정량에 사용한 결과 routine analyzer와 좋은 일치를 보였다.

A bio-sensor for the determination of glucose has been constructed by immobilizing the Citrobacter freundii or its organelle on carbon dioxide gas-sensor. The bacterial sensor was better than organelle in response, but the latter showed a shorter response time. The bacterial sensor gave linearity between 7.0 × 10-4 and 1.0 × 10-2 M glucose with a slope of 42.2 mV/decade in pH 7.0, 0.2 M tris-HCl buffer at 30℃. The selectivity of this sensor was very high for glucose. Employing for the determination of glucose in serum, the sensor showed a good agreement with a routine analyzer.

Page
424 - 429
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