ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 34, Number 5
JKCSEZ 34(5)
October 20, 1990 

A bio-sensor for the determination of glucose has been constructed by immobilizing the Citrobacter freundii or its organelle on carbon dioxide gas-sensor. The bacterial sensor was better than organelle in response, but the latter showed a shorter response time. The bacterial sensor gave linearity between 7.0 × 10-4 and 1.0 × 10-2 M glucose with a slope of 42.2 mV/decade in pH 7.0, 0.2 M tris-HCl buffer at 30℃. The selectivity of this sensor was very high for glucose. Employing for the determination of glucose in serum, the sensor showed a good agreement with a routine analyzer.