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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 32, Number 4
JKCSEZ 32(4)
August 20, 1988 

 
Title
Preparation and Comparison of Proteus mirabilis and Citrobacter freundii Bacterial Electrodes for the Determination of Cytosine

Cytosine 정량을 위한 Proteus mirabilis와 Citrobacter freundii 박테리아전극의 개발과 그 비교
Author
Gwon Shik Ihn, Bong Weon Kim

인권식, 김봉원
Keywords
Abstract
Cytosine에 대한 bio-electrode는 NH3 기체감응기에 Proteus mirabilis 와 Citrobacter freundii 박테리아를 고정하여 조립하였다. cytosine deaminase를 포함하는 박테리아는 cytosine 1분자를 NH3 1분자로 전환시킨다. Proteus mirabilis 박테리아 전극의 감응은 0.2M phosphate 완충용액, pH 8.4에서 1.0 × 10-3 - 7.0 × 10-3M직선범위와 45-48 mV/decade의 감응기울기를 가진다. Citrobacter freundii박테리아 전극의 감응은 0.05M phosphate완충용액, pH 7.6에서 7.0 × 10-5 - 7.0 × 10-3M 직선범위와 48 mV/decade의 감응기울기를 가진다. 이 전극을 pH, 온도, 완충용액, 박테리아의 양, 방해물질, 무기염류의 영향과 전극의 수명을 조사하였다.

The bio-electrode for cytosine has been constructed by immobilizing Proteus mirabilis and Citrobacter freundii on an ammonia gas-sensor. Bacteria containing cytosine deaminase convert one molecule of cytosine into one molecule of ammonia. The Proteus mirabilis bacterial electrode showed linear response to cytosine concentration in the 1.0 × 10-3 - 5.0 × 10-2M with a slope of 45-48 mV/decade in 0.2 M phospbate buffer solution at pH 8.4. The Citrobacter freundii bacterial electrode showed linear response to cytosine concentration in the 7.0 × 10-5 - 7.0 × 10-3M with a slope of 48 mV/decade in 0.05M phosphate buffer solution at pH 7.6. These electrode were investigated for the effects of pH, temperature, buffer solutions, amounts of bacteria, interferences, inorganic salts and lifetime.

Page
333 - 341
Full Text
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