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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 26, Number 4
JKCSEZ 26(4)
August 20, 1982 

 
Title
The Absorbance and Fluorescence of Chlorophyll-b in Organic Solvents (Ⅱ)

유기용매중에서 Chlorophyll-b의 흡광 및 형광 (제2보)
Author
Choong-Hwa Lee, Myong-Suk Kim, Koo-Chun Chung, Myon-Yong Park

이중화, 김명숙, 정구춘, 박면용
Keywords
Abstract
chl-b를 소중합체로서 용매에 녹였을 때 친핵성 n-prOH를 첨가함에 따라 흡광도와 fluorescence emission의 세기는 증가하였지만 단위체로 녹은 다음에 n-prOH의 농도증가에 따라 점차 감소하였다. chl-b는 소중합체의 생성때문에 Beer 법칙에 따르지 않았으며 용매의 극성변화에 따라 stockes shift를 나타냈다. 이러한 경향성은 chl-b의 정량에 고려하여야될 점이다.

The wavelength of chlorophyll-b on the absorbance and fluorescence emission were shifted to the longer depending on the increasing of solvent polarities but fluorescence excitation spectra were not. The presence of chl-b oligomers and monomers were identified by the specra of fluorescence emission. Fluorescence excitation, absorbance and the measurement of its intensities vs. the concentration of n-prOH added to chl-b solution. The calibration curve of chl-b solution were not obeyed to Beer's law in the range of concentrated soln. because of the presence as the oligomers.

Page
224 - 228
Full Text
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