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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 18, Number 2
JKCSEZ 18(2)
April 20, 1974 

 
Title
Variation of the Electrophoretic Mobility of Acid Mucopolysaccharides in Polyacrylamide Gel Electrophoresis

Polyacrylamide Gel Electrophoresis에 있어서 산성 Mucopolysaccharide의 이동도의 변화
Author
Kichang Kum, Julio J. Ludowieg

금기창, 줄리오 루도위-크
Keywords
Abstract
산성 mucopolysaccharide는 경태아의 nucleus pulposus에서 분리하였다. Gel electophoresis에 있어서 산성 mucopolysaccharide의 분리는 0.03%의 hexamine cobaltic chloride 를 함유하는 0.05M 초산소-다 완충용액(pH4.8)중에서 가장 좋았다. Spacer gel 상의 mucopolysaccharide 용액을 40% sucrose 용액으로 덮었을 때 mucopolysaccharide의 이동도에 변화가 나타나는 것을 관찰하였으며 이 효과는 초산소-다 완충용액에 hexamine cobaltic chloride 을 가하면 소실하는 것을 관찰하였다.

Acid mucopolysaccharides were isolated from nucleus pulposus of whale embryo. The separation of acid mucopolysaccharides was most excellent in 0.03 % hexamine cobaltic chloride in 0.05 M-sodium acetate buffer solution at pH 4.8. Changes in electrophoretic mobility of acid mucopolysaccharides were observed when the sample solution on top of spacer gel were covered with 40 % sucrose solution. This effect was not observed by the addition of hexamine cobaltic chloride to the buffer solution.

Page
132 - 137
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