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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 17, Number 3
JKCSEZ 17(3)
June 20, 1973 

 
Title
Effects of Nitrogen Sources in the Fermentation of Petroleum Hydrocarbon

석유탄화수소발효에 있어서의 질소원의 영향
Author
Tai Won Park, Taeyoung Kim, Hee Young Youn

박태원, 김태영, 윤희영
Keywords
Abstract
Rhodotorula sp.로 C12∼C14의 n-alkane 혼합물에서 무기계 질소원인 질산염과 암모늄염 그리고 유기계 질소원인 urea들의 그 화학형의 차이에 따라 발효시간과 수율면에서 어떻게 영향을 미치는가와 질산나트륨으로서 탄소원의 탄소에 대한 질소의 첨가비율의 차이에 따라 미치는 영향 등을 조사해 보았다. 그 결과 최고생장에 닫ㄹ하는데 요하는 시간은 질산나트륨과 황산암모늄이 각각 40 hrs.와 45hrs인데 비해 urea는 이들보다 훨씬 긴 66hrs.가 걸렸고 수율면에 있어서는 0.1N-NaOH의 소비량으로서 양무기염이 각각 0.36과 0.38ml인데 비해 urea는 0.78ml나 되었다. medium 중의 질소대 타소 비율의 영향은 medium에 가하는 n-alkane 혼합물을 1%(vol.)에서 질산나트륨으로서 N/C가 0.2일 때가 가장 우수한 효과를 나타내었고 대개 탄소에 대한 질소의 비율이 낮을 때보다는 높은 때가 더 좋은 생장효과를 보였다.

In the fermentation for preparation of petroprotein by Rhodotorula sp. in C12~C14 n-alkane mixture it was investigated how the effects due to the difference of chemical form of the nitrogen sources are, that is, how the inorganic nitrogen sources such as nitrates and ammonium salts and organic nitrogen source such as urea effect on the view of fermentation time and yield and how the ratios of nitrogen to carbon with NaNO3 effect. Then following results were obtained: the time required to maximum growth, when NaNO3 or (NH4)2SO4 was used as nitrogen source, was 40 hrs. and 45 hrs., respectively, but when urea was used, it was 66 hrs. much longer than above nitrogen sources. On the view of yield, however, in use of the both inorganic sources, when the yield is represented as consumption of 0.1 N-NaOH, it was 0.36 and 0.38 ml, respectively, but, in the case of urea, it amounted to 0.78 ml. In the effect of the ratios of nitrogen to carbon in medium, when n-alkane mixture was added in 1 % (vol.) and N/C with NaNO3 was 0.2 the best results were obtained and generally the higher the value of the ratio the better growth effects were shown.

Page
224 - 228
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