ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 17, Number 3
JKCSEZ 17(3)
June 20, 1973 

In the fermentation for preparation of petroprotein by Rhodotorula sp. in C12~C14 n-alkane mixture it was investigated how the effects due to the difference of chemical form of the nitrogen sources are, that is, how the inorganic nitrogen sources such as nitrates and ammonium salts and organic nitrogen source such as urea effect on the view of fermentation time and yield and how the ratios of nitrogen to carbon with NaNO3 effect. Then following results were obtained: the time required to maximum growth, when NaNO3 or (NH4)2SO4 was used as nitrogen source, was 40 hrs. and 45 hrs., respectively, but when urea was used, it was 66 hrs. much longer than above nitrogen sources. On the view of yield, however, in use of the both inorganic sources, when the yield is represented as consumption of 0.1 N-NaOH, it was 0.36 and 0.38 ml, respectively, but, in the case of urea, it amounted to 0.78 ml. In the effect of the ratios of nitrogen to carbon in medium, when n-alkane mixture was added in 1 % (vol.) and N/C with NaNO3 was 0.2 the best results were obtained and generally the higher the value of the ratio the better growth effects were shown.