ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 16, Number 5
JKCSEZ 16(5)
October 20, 1972 

The adduct between phenylglyoxal and guanine was prepared. The structure of the adduct is similar to the glyoxal-guanine adduct. Aldehyde group of phenylglyoxal is added to 1-N of guanine base, and keto group is added to N2 of guanine base. The structure of the adduct was determined by mass spectrometry, nuclear magnetic resonance spectroscopy and periodate oxidation. Periodate oxidation produced N2-benzoyl-guanine from the adduct. On the basis of these results, ittvhas been assigned the structure I. The adduct is stable in alkaline solution: It does not dissociate into phenylglyoxal and guanine even after 2 hours heating at 60℃ at pH 12. The adduct is soluble in acidic ethanol, and is slightly soluble in neutral or alkaline water. It has a lower A280/A260 ratio at pH 1 compared to that of guanine.