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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 15, Number 1
JKCSEZ 15(1)
February 20, 1971 

AN ESR STUDY OF AMINO ACID AND PROTEIN FREE RADICALS IN SOLUTION PART I. Reaction Mechanism of Free Radical Production in the Ti-H2O2 Flow System

용액에서의 아미노산 및 단백질 자유기에 관한 ESR 연구 제1보 ; Ti-H2O2 Flow System에서 자유기를 만드는 반응기구
Sun-Joo Hong, L. H. Piette

The reaction of amino acids and the reactive hydroxyl radical generated by Ti3+-H2O2 system was studied using fast flow techniques coupled with ESR. Upon adding methionine to the 0.2M H2O2 solution (0.05M methionine after addition) and mixing with 0.01M TiCl3, the low field component of the two incompletely resolved peaks, in the spectrum of Ti3+-H2O2 system alone, vanished completely whereas the high field component remained almost constant and superimposed on the secondary spectrum of the methionine free radical. Similar results were obtained for other amino acids and proteins. The results strongly demonstrate that the T3+-H2O2 flow system generates two different radical species, only one of which, giving rise to the low field component, is alone responsible for abstracting hydrogen atoms from substrate molecules. The effects of HCl, H2SO4 and NaOH on the system were also studied with widely varying results.
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