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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 5, Number 1
February 20, 1961 

Unknown Nitrogen Compound in Germinating Mung Bean

(제2보) 발아녹두중의 미지질소화합물에 관하여 (Part 2)
Tae Rin Kim, Chang Won Song

김태인, 송창원
A ninhydrin positive substance(formed during germination of Mung Bean) which on a 2-dimensional chromatography(BuOH-HAc, and PhOH-NH2 gave a spot above glutamic acid was isolated by cutting out the appropriate spot on the paper and extracting the paper with water. Hydrolysis of this substance with 2N H2SO4 or 3N HCl at 120℃ for 15 hours gave the spot of mainly glutamic acid, alanine and very faint of glycine and cysteine on a paper chromatography. It is suggested that the reaction of γ-glutamyl-cysteinecyl-glycine + L-alanine in the presence of γ-glutamyle transferase → γ-glutamyl-alanine + cysteinyl-glycine, takes place in germinated Mung Bean.
15 - 17
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