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ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 65, Number 4
JKCSEZ 65(4)
August 20, 2021 |
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Title |
Simultaneous Spectrometric Determination of Caffeic Acid, Gallic Acid, and Quercetin in Some Aromatic Herbs, Using Chemometric Tools
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Author |
Abdelmalek Kachbi*, Dalila Abdelfettah Kara, Mohamed Benamor, Ounissa Senhadji Kebiche* |
Keywords |
Simultaneous determination, Chemometrics, Phenolic compounds, UV-visible, Herbs
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Abstract |
The purpose of this work is the development of a method for an effective, less expensive, rapid, and simultaneous determination of three phenolic compounds (caffeic acid, gallic acid, and quercetin) widely present in food resources and known for their antioxidant powers. The method relies on partial least squares (PLS) calibration of UV-visible spectroscopic data. This model was applied to simultaneously determine, the concentrations of caffeic acid (CA), gallic acid (GA), and quercetin (Q) in six herb infusion extracts: basil, chive, laurel, mint, parsley, and thyme. A wavelength range (250-400) nm, and an experimental calibration matrix with 21 samples of ternary mixtures composed of CA (6.0-21.0 mg/L), GA (10.0-35.2 mg/L), and Q (6.4-17.5 mg/L) were chosen. Spectroscopic data were mean-centered before calibration. Two latent variables were determined using the contiguous block cross-validation procedure after calculating the root mean square error cross-validation RMSECV. Other statistic parameters: RMSEP, R2, and Recovery (%) were used to determine the predictive ability of the model. The results obtained demonstrated that UV-visible spectrometry and PLS regression were successfully applied to simultaneously quantify the three phenolic compounds in synthetic ternary mixtures. Moreover, the concentrations of CA, GA and Q in herb infusion extracts were easily predicted and found to be 3.918?18.055, 9.014?23.825, and 9.040?13.350 mg/g of dry sample, respectively.
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Page |
254 - 259 |
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