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Journal of the Korean Chemical Society (JKCS)

ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 51, Number 5
JKCSEZ 51(5)
October 20, 2007 

 
Title
Measuring Oxytetracycline Using a Simple Prepared DNA Immobilized on a Carbon Nanotube Paste Electrode in Fish Tissue

DNA 고정 탄소나노튜브 페이스트전극의 물고기 세포속 테트라싸이클린에 정량
Author
Suw Young Ly*, Chang Hyun Lee, Young Sam Jung

이수영*, 이장현, 정영삼
Keywords
Oxytetracycline, Square Wave Stripping, Cyclic Voltammetry, Carbon Nanotube, DNA, Paste Electrode , Oxytetracycline, Square Wave Stripping, Cyclic Voltammetry, Carbon Nanotube, DNA, Paste Electrode
Abstract
DNA를 고정한 탄소나노튜브 페이스트 전극으로 옥시테트라싸이클린 항생제의 분석조건을 연구하였다. 사각 파형 벗김 전압전류법과 순환 전압 전류법을 사용하여 최적조건에서 1-10 ngL?1의 정량 가능 범위에 도달하였다. 벗김법 0.1 mgL?1 농도에서 15번 반복 측정하여 0.068%의 상대표준편차였으며, 벗김법 최적 분석 조건에서 0.4 ngL?1의 검출 한계를 얻었다. 개발된 결과를 양식어류의 세포에서 응용하였다.

A simple prepared paste electrode (PE) of DNA immobilized on a carbon nanotube was utilized for monitoring the antibacterial agent oxytetracycline (OTC), using square-wave anodic stripping voltammetry (SWASV) and cyclic voltammetry (CV). Given these conditions, SWASV and CV working ranges were observed within 1-10 ngL-1 OTC. In the SWASV and CV for OTC concentrations of 0.1 mgL-1, the relative standard deviations (n=15) were 0.068 and 0.067, respectively. At the optimized condition, the detection limit was found to be 0.4 ngL-1 OTC. This method was applied to the hatchery fish tissue.

Page
412 - 417
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