ISSN 1017-2548(Print)
ISSN 2234-8530(Online)
Volume 49, Number 2
JKCSEZ 49(2)
April 20, 2005 

The extracts of the whole herb of Artemisia princeps PAMPAN have been used for the treatment of inflammation, cancer and other disorders. In this experiment to extract and separate eupatilin contained in Artemisia princeps PAMPAN using a preparative chromatography, the optimum operating conditions were experimentally determined. eupatilin was extracted from the leaf and trunk of Artemisia princeps PAMPAN by ethanol. The resulting solution was further partitioned with n-hexane, chloroform and ethyl acetate. The mobile phase composition and the injection volume were water/ acetonitrile/TFA=50/50/0.5 (vol. %) and 20 ml, respectively. The flow rate was fixed at l.0 ml/min and UV wavelength was 370 nm. The measured retention time of eupatilin was approximately 8.6 min. The solution which contained eupatilin was collected by preparative and analytical column, and was identificated by LC-CE-MS.