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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 18, Number 11
BKCSDE 18(11)
November 20, 1997

Title	Effect of Glycerophospholipid on Oleate-Dependent Phospholipase D in Mammalian Source
Author	Myung Joo Kim, Bong-Sub Kim, Sang Yoon Lee, Jeong Won Sohn, Chul Yong Kim, Myung-Sun Choi, Myung-Un Choi*
Keywords
Abstract	Effects of exogenous glycerophospholipids on oleate-dependent phospholipase D (PLD) activity were studied in lymphocytic mouse leukemia L1210 cells and in solubilized microsomal phospholipase D of rat brain. Among the phospholipids tested phosphatidic acid had the most stimulatory effects on both PLD activities up to about 3 folds. Lysophosphatidic acid also showed promoting effect on microsomal PLD activity but much less on L1210 cells compared to that of phosphatidic acid. While phosphatidylethanolamine increased PLD activity slightly, phosphatidylinositides were nearly ineffective in the tested sources. The stimulatory effect of phosphatidic acid observed can be utilized to improve the in vitro assay system for oleate-dependent PLD in mammalian sources.
Page	1204 - 1207
Full Text	PDF

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