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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 16, Number 8
BKCSDE 16(8)
August 20, 1995 

Kinetic Mechanism of Cellulose Hydrolysis by Endoglucanase Ⅰ and Exoglucanase Ⅱ Purified from Trichoderma viride
Dong Won Kim*, Young Kyu Jeong, Young Hun Jang, Jae Kuk Lee, Ki Sung Kim, Haiil Ryu
A kinetic study of the hydrolysis of celluloses with different crystallinity by Endo Ⅰ and Exo Ⅱ purified from Trichoderma viride cellulase was performed. It was found that the hydrolysis of the celluloses can be described by a summation of two parallel pseudo first order reactions. From the experimental data, it is possible to calculate the fractions of easily and difficult hydrolyzable cellulose in Sigmacell 100 and associated rate constants. As the results, all of the rate constants decreased with increasing crystallinity of cellulose. From the kinetic data, it was also found that the coexistence of Endo Ⅰ and Exo Ⅱ showed a synergistic effect in the latter phase of the enzymatic hydrolysis reaction.
742 - 747
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